Genome of Methylobacillus flagellatus , Molecular Basis for Obligate Methylotrophy, and Polyphyletic Origin of Methylotrophy
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چکیده
منابع مشابه
Genome of Methylobacillus flagellatus, molecular basis for obligate methylotrophy, and polyphyletic origin of methylotrophy.
Along with methane, methanol and methylated amines represent important biogenic atmospheric constituents; thus, not only methanotrophs but also nonmethanotrophic methylotrophs play a significant role in global carbon cycling. The complete genome of a model obligate methanol and methylamine utilizer, Methylobacillus flagellatus (strain KT) was sequenced. The genome is represented by a single cir...
متن کاملThe expressed genome of Methylobacillus flagellatus as 1 defined through comprehensive proteomics and new 2 insights into methylotrophy 3 4
4 Erik L. Hendrickson, David A. C. Beck, Tiansong Wang, Mary E. Lidstrom, Murray 5 Hackett, Ludmila Chistoserdova 6 7 Department of Chemical Engineering, University of Washington, Box 355014, Seattle, 8 WA 98195, USA 9 10 2 The eScience Institute, University of Washington, Box 355014, Seattle, WA 98195, 11 USA 12 Department of Microbiology, University of Washington, Box 355014, Seattle, WA 9819...
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Methanol is increasingly becoming an attractive substrate for production of different metabolites, such as commodity chemicals, and biofuels via biological conversion, due to the increment of annual production capacity and decrement of prices. In recent years, genetic engineering towards native menthol utilizing organisms – methylotrophy has developed rapidly and attracted widespread attention....
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The isocitrate dehydrogenase (MfIDH) with unique double coenzyme specificity from Methylobacillus flagellatus was purified and characterized, and its gene was cloned and overexpressed in E. coli as a fused protein. This enzyme is homodimeric,-with a subunit molecular mass of 45 kDa and a specific activity of 182 U mg -1 with NAD+ and 63 U mg -1 with NADP+. The MfIDH activity was dependent on di...
متن کاملOrganization of threonine biosynthesis genes from the obligate methylotroph Methylobacillus flagellatus.
The genes encoding aspartate kinase (ask), homoserine dehydrogenase (hom), homoserine kinase (thrB) and threonine synthase (thrC) from the obligate methylotroph Methylobacillus flagellatus were cloned. In maxicells hom and thrC directed synthesis of 51 and 48 kDa polypeptides, respectively. The hom, thrB and thrC genes and adjacent DNA areas were sequenced. Of the threonine biosynthesis genes, ...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2007
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.00045-07